Protein a-enzyme monoconjugate as a versatile tool for enzyme immunoassays

A Recyclable Poly(ionic liquid)s Enzyme Reactor for Highly Efficient Protein Digestion

One of the most significant tasks for proteomic research and industrial applications, is the preparation of recyclable enzyme reactor. Herein, a novel recyclable enzyme reactor has been developed based on monodispersed spherical poly(quaternary ammonium ionic liquid)s particles immobilized trypsin. A new quaternary ammonium ionic liquids functional monomer was first synthesized. The ionic l...

Inositol Phosphates: A Remarkably Versatile Enzyme

Ins(3,4,5,6)P(4) is an inhibitor of Ca(2+)-activated Cl(-) channels, but further understanding has been hindered by ignorance of how it is made in cells. It now transpires that one protein with ATP-dependent kinase and phosphatase activities interconverts Ins(3,4,5,6)P(4) and Ins(1,3,4,5,6)P(5), as well as several other inositol polyphosphates.

Streptavidin - A Versatile Binding Protein for Solid-Phase Immunoassays

A Physico-Mathematical Model for Bisubstrate Enzyme Membrane Electrodes

A physic-mathematical model has been developed that is suitable for biosensors incorporating membranes and bisubstrate enzymes. The set of equations regarding the transport of reactants across the membrane, the bisubstrate enzymatic reaction, back diffusion of reactants and reaction products, are written down and simultaneously solved and transient response of the electrode is determined as...

Enzyme immunoassays as a method for quantifying hair reproductive hormones in two felid species

Non-invasive monitoring of wild felid reproductive states is important, given that many species reproduce poorly in captivity. Despite extensive work in faecal hormone analysis in felids, continued development of techniques is necessary, particularly with wild populations. In this study, our aims were as follows: (i) biochemical validation of enzyme immunoassays for estrogen, testosterone and p...